Culture dependent vs culture independent
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Culture Dependent Vs Culture Independent. Comparing the culture dependent and culture independent approaches. CIDTs can provide results in as little as a few hours and can even be performed in-house. These tests often require less expensive equipment and less training for the test to. Coral Reefs 32 375388 2013.
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Here we compared culture and culture-independent molecular methods in characterizing the vaginal microbiota in Indian women. Comparing the culture dependent and culture independent approaches. The purpose of this study is to use culture-dependent and culture-independent means of microtyping to determine whether any association exists between the quantity and quality of bacteria identified in patients with recalcitrant chronic rhinosinusitis. Culture-dependent methods for diversity studies. A paradigm in microbial ecology is that culture-based techniques only recover 1-10 of the true bacterial diversity within an environment 29 30 and that molecular surveys of bacterial communities yield dramatically different results than traditional culture approaches. Culture-independent methods revealed greater numbers and greater richness of bacteria on apple leaves than found by culturing.
Culture-independent methods have been proposed to provide a less biased picture of the richness of bacterial communities than culture-dependent methods 2 3 24 because of the selective pressure imposed by the requirement of the latter for growth on a solid substrate leading to the isolation of a plate-growth-adapted subpopulation from the.
The purpose of this study is to use culture-dependent and culture-independent means of microtyping to determine whether any association exists between the quantity and quality of bacteria identified in patients with recalcitrant chronic rhinosinusitis. A comparison of culture-dependent and culture-independent techniques used to characterize bacterial communities on healthy and white plague-diseased corals of the Montastraea annularis species complex. Lets take a look at the differences between CIDTs and culture confirmation tests. This is the first study to directly compare culture-dependent and independent approaches for assessing bacterial communities in the phyllosphere. The purpose of this study is to use culture-dependent and culture-independent means of microtyping to determine whether any association exists between the quantity and quality of bacteria identified in patients with recalcitrant chronic rhinosinusitis. Culture-independent methods revealed greater numbers and greater richness of bacteria on apple leaves than found by culturing.
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Picual or by xylem sap extraction method Scholander chamber SCh or wood chips WC maceration. Novel high-throughput culture-independent techniques hold promise but have not been systematically compared to conventional culture. Culture-Independent Methods - requires cell stains to obtain data and does not rely on culturing microorganisms Culture-Dependent Methods - needs to culture the microorganism 1. While culture-independent pyrosequencing successfully identified bacterial communities in a greater percentage of specimens conventional bacterial culture was largely successful in identifying the dominant microbe present in instances of acute infection as confirmed via novel culture-independent indices of acute infection high total bacterial DNA burden low bacterial community diversi. It is called dependent because it depends on the independent variable.
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A comparison of culture-dependent and culture-independent techniques used to characterize bacterial communities on healthy and white plague-diseased corals of the Montastraea annularis species complex. Culture-Independent Methods - requires cell stains to obtain data and does not rely on culturing microorganisms Culture-Dependent Methods - needs to culture the microorganism 1. These tests often require less expensive equipment and less training for the test to. Medical records were retrospectively reviewed for patients with a history of. This is the first study to directly compare culture-dependent and independent approaches for assessing bacterial communities in the phyllosphere.
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A dependent variable responds to changes in the independent variable Babbie 2007. Culture-independent methods have been proposed to provide a less biased picture of the richness of bacterial communities than culture-dependent methods 2 3 24 because of the selective pressure imposed by the requirement of the latter for growth on a solid substrate leading to the isolation of a plate-growth-adapted subpopulation from the. Although molecular approaches such as 16S rDNA amplicon or whole metagenome shotgun WMS sequencing provide some clear benefits compared with culture-dependent methods by reason of their ability to provide direct and in-depth insights into the composition of the microbiota in a culture-independent manner they seem to lack in the detection of low-abundant organisms. Methods for investigating microbiota from a given ecosystem can be either culture-dependent or culture-independent. They are known to be biased because bacteria can only be cultivated if their metabolic and physiological requirements can be reproduced in vitro Nadkarni et al 2009.
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A comparison of culture-dependent and culture-independent techniques used to characterize bacterial communities on healthy and white plague-diseased corals of the Montastraea annularis species complex. Culture-Independent Methods - requires cell stains to obtain data and does not rely on culturing microorganisms Culture-Dependent Methods - needs to culture the microorganism 1. Culture-independent methods revealed greater numbers and greater richness of bacteria on apple leaves than found by culturing. Lets take a look at the differences between CIDTs and culture confirmation tests. A dependent variable responds to changes in the independent variable Babbie 2007.
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Coral Reefs 32 375388 2013. Comparing the culture dependent and culture independent approaches. Significance and Impact of the Study. Although molecular approaches such as 16S rDNA amplicon or whole metagenome shotgun WMS sequencing provide some clear benefits compared with culture-dependent methods by reason of their ability to provide direct and in-depth insights into the composition of the microbiota in a culture-independent manner they seem to lack in the detection of low-abundant organisms. We analyzed 46 clinically obtained bronchoalveolar lavage BAL fluid specimens from symptomatic and asymptomatic lung transplant recipients both by culture using a clinical microbiology laboratory protocol and by bacterial 16S rRNA gene pyrosequencing.
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A dependent variable responds to changes in the independent variable Babbie 2007. Novel high-throughput culture-independent techniques hold promise but have not been systematically compared to conventional culture. Picual or by xylem sap extraction method Scholander chamber SCh or wood chips WC maceration. Here we compared culture and culture-independent molecular methods in characterizing the vaginal microbiota in Indian women. A dependent variable responds to changes in the independent variable Babbie 2007.
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Culture-dependent methods present several disadvantages above all for determining bacterial numbers. The present study focused on i the comparison of a culture-dependent approach culturing applying identification using morphological and phylogenetic analysis of multiple genes based identification versus a culture-independent approach meta-barcoding of the fungal ITS rDNA barcode for characterization of diversity and community composition of fungal endophytes associated with stems of grapevines. Figure S5 Hierarchical cluster analysis and heat-map of the relative abundance of bacterial genera obtained using culture-dependent approaches left panel or culture-independent approach right panel in olive xylem sap samples when compared by olive genotype Ace. The biases introduced by different methods will have a. This is the first study to directly compare culture-dependent and independent approaches for assessing bacterial communities in the phyllosphere.
Source: microbeonline.com
What are some culture-independent methods. Figure S5 Hierarchical cluster analysis and heat-map of the relative abundance of bacterial genera obtained using culture-dependent approaches left panel or culture-independent approach right panel in olive xylem sap samples when compared by olive genotype Ace. The present study focused on i the comparison of a culture-dependent approach culturing applying identification using morphological and phylogenetic analysis of multiple genes based identification versus a culture-independent approach meta-barcoding of the fungal ITS rDNA barcode for characterization of diversity and community composition of fungal endophytes associated with stems of grapevines. Culture-dependent methods for diversity studies. This is the first study to directly compare culture-dependent and independent approaches for assessing bacterial communities in the phyllosphere.
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Here we compared culture and culture-independent molecular methods in characterizing the vaginal microbiota in Indian women. Lets take a look at the differences between CIDTs and culture confirmation tests. Culture-Dependent and Culture-Independent Characterization of Microbial Assemblages Associated with High-Temperature Petroleum Reservoirs V. What is the difference between culture-independent vs. We analyzed 46 clinically obtained bronchoalveolar lavage BAL fluid specimens from symptomatic and asymptomatic lung transplant recipients both by culture using a clinical microbiology laboratory protocol and by bacterial 16S rRNA gene pyrosequencing.
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We analyzed 46 clinically obtained bronchoalveolar lavage BAL fluid specimens from symptomatic and asymptomatic lung transplant recipients both by culture using a clinical microbiology laboratory protocol and by bacterial 16S rRNA gene pyrosequencing. The present study focused on i the comparison of a culture-dependent approach culturing applying identification using morphological and phylogenetic analysis of multiple genes based identification versus a culture-independent approach meta-barcoding of the fungal ITS rDNA barcode for characterization of diversity and community composition of fungal endophytes associated with stems of grapevines. Medical records were retrospectively reviewed for patients with a history of. We analyzed 46 clinically obtained bronchoalveolar lavage BAL fluid specimens from symptomatic and asymptomatic lung transplant recipients both by culture using a clinical microbiology laboratory protocol and by bacterial 16S rRNA gene pyrosequencing. Culture-independent methods revealed greater numbers and greater richness of bacteria on apple leaves than found by culturing.
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Culture-dependent methods present several disadvantages above all for determining bacterial numbers. While culture-independent pyrosequencing successfully identified bacterial communities in a greater percentage of specimens conventional bacterial culture was largely successful in identifying the dominant microbe present in instances of acute infection as confirmed via novel culture-independent indices of acute infection high total bacterial DNA burden low bacterial community diversi. It is called dependent because it depends on the independent variable. These tests often require less expensive equipment and less training for the test to. What is the difference between culture-independent vs.
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We analyzed 46 clinically obtained bronchoalveolar lavage BAL fluid specimens from symptomatic and asymptomatic lung transplant recipients both by culture using a clinical microbiology laboratory protocol and by bacterial 16S rRNA gene pyrosequencing. Although molecular approaches such as 16S rDNA amplicon or whole metagenome shotgun WMS sequencing provide some clear benefits compared with culture-dependent methods by reason of their ability to provide direct and in-depth insights into the composition of the microbiota in a culture-independent manner they seem to lack in the detection of low-abundant organisms. The purpose of this study is to use culture-dependent and culture-independent means of microtyping to determine whether any association exists between the quantity and quality of bacteria identified in patients with recalcitrant chronic rhinosinusitis. These tests often require less expensive equipment and less training for the test to. Novel high-throughput culture-independent techniques hold promise but have not been systematically compared to conventional culture.
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Culture-dependent methods present several disadvantages above all for determining bacterial numbers. Culture-dependent methods present several disadvantages above all for determining bacterial numbers. CIDTs can provide results in as little as a few hours and can even be performed in-house. Medical records were retrospectively reviewed for patients with a history of. What is the difference between culture-independent vs.
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Problems with using culturing. The purpose of this study is to use culture-dependent and culture-independent means of microtyping to determine whether any association exists between the quantity and quality of bacteria identified in patients with recalcitrant chronic rhinosinusitis. What are some culture-independent methods. A paradigm in microbial ecology is that culture-based techniques only recover 1-10 of the true bacterial diversity within an environment 29 30 and that molecular surveys of bacterial communities yield dramatically different results than traditional culture approaches. Novel high-throughput culture-independent techniques hold promise but have not been systematically compared to conventional culture.
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What is the difference between culture-independent vs. A dependent variable is the variable that is measured in an experiment Babbie 2007. Culture methods involved traditional cultivation on Rogosa and sheep blood agar whereas culture-independent methods bypassed a culturing step by performing broadrange 16S rDNA PCR on DNA isolated directly from vaginal swabs. Coral Reefs 32 375388 2013. This is the first study to directly compare culture-dependent and independent approaches for assessing bacterial communities in the phyllosphere.
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Picual or by xylem sap extraction method Scholander chamber SCh or wood chips WC maceration. Medical records were retrospectively reviewed for patients with a history of. Following independent design and stable production this deals with a culture of interdependence in support of a delivery strategy. What are some culture-independent methods. However although the gap between culture-independent and culture-dependent analyses of soil microbial communities is often mentioned few studies 36 38 have directly compared these approaches and none has specifically investigated the biases associated with culturing both bacteria and fungi in a bioremediation context despite the critical role of culturing in this field.
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While culture-independent pyrosequencing successfully identified bacterial communities in a greater percentage of specimens conventional bacterial culture was largely successful in identifying the dominant microbe present in instances of acute infection as confirmed via novel culture-independent indices of acute infection high total bacterial DNA burden low bacterial community diversi. While culture-independent pyrosequencing successfully identified bacterial communities in a greater percentage of specimens conventional bacterial culture was largely successful in identifying the dominant microbe present in instances of acute infection as confirmed via novel culture-independent indices of acute infection high total bacterial DNA burden low bacterial community diversi. A dependent variable is the variable that is measured in an experiment Babbie 2007. The present study focused on i the comparison of a culture-dependent approach culturing applying identification using morphological and phylogenetic analysis of multiple genes based identification versus a culture-independent approach meta-barcoding of the fungal ITS rDNA barcode for characterization of diversity and community composition of fungal endophytes associated with stems of grapevines. Coral Reefs 32 375388 2013.
Source: researchgate.net
What are some culture-independent methods. These tests often require less expensive equipment and less training for the test to. Here we compared culture and culture-independent molecular methods in characterizing the vaginal microbiota in Indian women. Culture-dependent methods for diversity studies. Comparing the culture dependent and culture independent approaches.
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